Herbicide-resistant rice plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

Patent

Brief

Patent brief

Problem

How can battery fires and thermal runaway in EV packs be prevented?

Novelty

Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and h...

Uses

Patent review

Assignee

INSTITUTO NACIONAL DE TECHNOLOGIA AGROPECUARIA

Published

Mar 10, 2026

Inventors

Alberto Livore, Alberto Raul Prina, Bijay K. Singh, Robert Ascenzi, Sherry R. Whitt

Domain

Mobility / storage

Plain-English summary

Problem

How can battery fires and thermal runaway in EV packs be prevented?

Solution

Key novelty

Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described.

Applications

Patent review

Relevant search intents

62b4ba0476a0

Claims preview

1 . A viable non-transgenic rice seed, comprising: a mutagenized endogenous rice acetohydroxyacid synthase large subunit (AHASL1) polynucleotide having a sequence that encodes an herbicide-tolerant AHASL1 protein comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:2 having a valine at position 179, wherein the polynucleotide is free of site-directed mutations; and a phenotype of tolerance to 75 cc active ingredient/hectare (a.i./ha) imazapyr and 24.85 g a.i./ha imazapic, as a result of the presence of the valine at position 179 of SEQ ID NO:2, wherein said sequence of the polynucleotide has been obtained by chemical mutagenesis using a chemical mutagen. 2 . The viable non-transgenic seed of claim 1 , said rice AHASL1 polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1 or 3. 3 . A viable non-transgenic rice seed, comprising: a mutagenized endogenous rice acetohydroxyacid synthase large subunit (AHASL1) polynucleotide having a sequence that encodes a herbicide-tolerant AHASL1 protein comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:2 having a valine at position 179, wherein the polynucleotide is free of sited-directed mutations; and a phenotype of tolerance to 75 cc active ingredient/hectare (a.i./ha) imazapyr and 24.85 g a.i./ha imazapic as a result of the presence of the valine at position 179 of SEQ ID NO:2, wherein said sequence of the polynucleotide has been obtained by chemical mutagenesis using a chemical mutagen, and wherein said herbicide-tolerant AHASL1 protein further comprises at least one member selected from the group consisting of: (a) a threonine at amino acid position 96 of SEQ ID NO:2; (b) an alanine, threonine, histidine, leucine, arginine, isoleucine, glutamine, or serine at amino acid position 171 of SEQ ID NO:2; (c) a leucine at amino acid position 548 of SEQ ID NO:2; and (d) an asparagine, threonine, or phenylalanine at amino acid position 627 of SEQ ID NO:2.

Herbicide-resistant rice plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use

Plain-English summary

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